Figure 6

Semi-quantitation RT-PCR analysis of genes up-regulated (A) and down-regulated (B) in HeLa Tet-off cells over-expressing TSPY, using gene-specific primers (Table 1). C = cells stably transfected with vector (pTIG) alone; T = cells stably transfected with TIG-TSPY. The relative expression was calculated by digitization and quantitation software (LabWorks, UVP Inc.). The ratio of T/C represents the relative expression level between the TSPY expressing and control cells for the respective genes (see Table 2).