Figure 1From: Differential regulation of NF-κB activation and function by topoisomerase II inhibitorsInduction NF-κB DNA binding by topoisomerase II inhibitors and DNA-intercalators. U-2 OS cells were stimulated with 1 μM aclarubicin, 1 μM daunorubicin, 1 μM doxorubicin, 2.5 μM mitoxantrone and 4 μM ICRF-193, for the times indicated. Nuclear protein extracts were prepared and 5 μg was subjected to EMSA analysis using a 32P labelled HIV-1 κB oligonucleotide.Back to article page