Figure 2

Levels of mRNA of various thyroid specific genes in wild-type and transfected ARO cells. Total RNA was extracted from duplicate dishes of wild-type ARO cells (ARO), cells containing the empty vector (ARO-pCMV), two selected clones expressing Pax8 gene (ARO-Pax8 cl. 8 and ARO-Pax8 cl. 21) and the levels of RNA were determined using the real-time PCR method. To normalize the different amount of total RNA added to the reaction, an amplification of GAPDH or β-actin was performed as endogenous control. The data are expressed as the mean ± SD of values obtained from at least three different experiments. Statistic analysis was performed using the one-factor ANOVA, followed by t test. P < 0.05 was considered statistically significant.