Figure 5

Distinctive regulation of NF-kB activation in MCF7 breast cancer cells by Ad5hTRAIL and/or AdIKKβKA infections. MCF7 cells were simultaneously infected with AdNFkBLuc, Ad5hTRAIL and/or increasing doses of AdIKKβKA construct for 24 hours. AdCMVLacZ infection was also performed as a negative control. The types of constructs used in the infection are shown on the x axis. MOI values represent DNA particles/cell. Ad5hTRAIL vector was used at two different constant MOIs (MOI of 1000 and 5000) in order to avoid cell death complicating our assay result. Luciferase activity expressed in Relative Light Units per microgram protein is shown on y axis. Values represent the mean (± SEM) of six independent data points (n = 6).