Name | Relevant characteristics/sequence | Source or reference |
---|---|---|
Bacterial strains: | ||
E. coli DH5α | F-, ø80dlacZΔM15, Δ(lacZYA-argF)U169 deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Takaba |
Salmonella enterica serovar | ||
Typhimurium aroA SL7207 | hisG46, DEL407 [aroA544::Tn10 (Tcs)] | Stocker, B. A. D. |
Salmonella enterica serovar | ||
Typhimurium LB5000 | rk-, mk+ | Stocker, B. A. D. |
Plasmids: | ||
pUC13-c-raf-1 | c-raf cDNA in pUC13 | [12] |
pcDNA3 | pCMV, ApR, Neomycin, SV 40, ColE | Invitrogene |
pcDNA-craf | craf cDNA in pcDNA3 | Troppmair, J |
pMOhly1 | ApR, hlyR, hlyC, hlyAs (encoding the hemolysin secretion signal), hlyB, hlyD | [13] |
pMOhly-Raf | ApR, hlyR, hlyC, raf-hlyAs (encoding a C-Raf-hemolysin fusion), hlyB, hlyD | this study |
Cell lines: | ||
EL-4 | spontaneous murine lymphoma | ATCC(Rockville, MD, USA) |
EL-4Raf | pcDNA-craf transfected EL-4 cells | this study |
SF9 | insect cell line | Gibco |
SF9 Raf | craf transfected SF9 cells | [28] |
Mice: | ||
C57BL/6JolaHsd | wild-type (H-2b) | Harlan-Winkelmann, Borche, Germany |
BxB23 | [(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter | [15] |
BxB11 | [(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter | [15] |