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Figure 8 | BMC Cancer

Figure 8

From: Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression

Figure 8

Neuroblastoma and pre-B lymphoma differs in expression of CRE and Pu.1 site binding proteins. Panel (A) display EMSA analysis of nuclear extracts from pre-B (KM3) or neuroblastoma cells (SH-SY5Y and SK-N-BE(2)c) incubated with a consensus cAMP responsive element (CRE). The two major complexes are denoted ATFC1 and ATFC2. Panel (B) show an EMSA analysis using nuclear extracts from either pre-B lymphoma cells (Nalm6) or the neuroblastoma cells SH-SY5Y and SK-N-BE(2)c, incubated with a labelled Pu.1 binding site from the mouse Immunoglobulin l enhancer (lB). Ets1/2 or PU.1 antibody was included in the reactions as indicated. The arrow indicates the complex super-shifted by the anti PU.1 antibody.

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BMC Cancer

ISSN: 1471-2407

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