Figure 7
Effects of growth factors on the cell cycle distribution and extent of senescence of siRNA-treated Kasumi-1 cellsA. Autocrine G-CSF expression in dependence on the siRNA treatment. Kasumi-1 cells were electroporated at days 0 and 4. Total RNA was isolated on day 8 and analyzed by real-time RT-PCR. The columns and error bars represent the means and standard deviations of three independent experiments. B. Cell cycle distribution of siRNA-treated cells in the absence and presence of growth factors. Kasumi-1 cells were analyzed on day 8 by FACS analysis as described in Materials and Methods. C. Amount of S phase cells in dependence on the length of RUNX1-CBFA2T1 depletion. D. Amount of senescent cells dependence on the length of RUNX1-CBFA2T1 depletion. Growth factors are indicated below the graphs. The data shown in B, C and D were obtained from the same time course experiment. Cells were electroporated with the indicated siRNAs at days 0, 4, 8 and 12, and were analyzed on days 4, 8, 12 and 16.