Figure 4
RUNX1-CBFA2T1 depletion inhibits cell proliferation and G1-S transition in Kasumi-1A. Proliferation curve of siRNA treated cells. Kasumi-1 cells were electroporated at days 0, 4, 8 and 12. Cell numbers were determined at the indicated days using trypan blue counting. Arrows indicate electroporations. B. Graphical representation of cell doubling times. The columns represent the means of three independent experiments. Error bars indicate standard deviations. *, p < 0.05 according to Student's t-test. C. Graphical representation of the cell cycle phase distribution. Kasumi-1 cells were electroporated at days 0 and 4, and were examined at day 8 using FACS analysis. The columns and error bars represent the mean values and standard deviations of three independent experiments. †, p < 0.01 according to Student's t-test. C. RUNX1-CBFA2T1 suppression is associated with increased CDKN1B (p27Kip1) levels. After electroporation at days 0 and 4, total cell lysates were prepared at day 8 and analyzed using immunoblotting. After CDKN1B detection, the membrane was stripped and reprobed with an anti-tubulin antibody. The siRNAs are indicated on top. Arrows on the left mark RUNX1-CBFA2T1, RUNX1, CDKN1B (p27Kip1) and tubulin proteins. The relative ratios between CDKN1B and tubulin are indicated below the blot.