Figure 2

DARC-A549 transfectants have increased expression of DARC protein and an increased ability to bind IL-8. A) The expression of DARC by transfected cells was determined by FACS analysis using the DARC-specific antibody Fy6. We saw no increase in the fluorescent intensity of the Control cell line following addition of the Fy6 antibody (solid black line) as compared to IgG isotype control (dotted black line). For both DARC1A6 (light gray line) and DARC2F5 (dark gray line) we observed an increase in the fluorescent intensity following addition of Fy6 antibody suggesting the expression of the protein on the surface of DARC-transfected cells. B) We observed an increase in IL-8 binding in both DARC1A6 (light gray line) and DARC2F5 (dark gray line) as compared to the Control transfected A549 cell line (black line), indicating increased and functional ligand binding of IL-8 in DARC-expressing cell lines.