Figure 5
Induction of cell cycle arrest by IFNγ and MIBG After 48 h of incubation IFNγ dose-dependently induced an arrest in the S-phase of the cell cycle of BON cells. Accordingly, the proportion of cells in the G2/M-phase decreased. Differences in the proportion of cells in the respective phase of cell cycle versus control were significant from 250 to 500 U/ml IFNγ (p < 0.05). Incubation of BON cells with MIBG for 48 h induced a dose-dependent arrest in the G0/G1-phase, while the proportion of cells in the G2/M-phase decreased. Differences in the proportion of cells in the respective phase of cell cycle versus control were significant at 10 and 50 μM MIBG (p<0.05). Combination treatment of MIBG and IFNγ abolished MIBG-induced G0/G1-phase arrest. Means of 4 independent experiments are shown.