Table 1 Primer sequences used for RT-PCR reactions
From: Gene expression profile of AIDS-related Kaposi's sarcoma
Gene/fragment size | Forward primer 5'-3' | Reverse primer 5'-3' |
|---|---|---|
Dual specificity phosphatase: outer set | AGT GGG TAC ATC AAG TCC ATC TGA | CAC TGG TAT TTT CCA TCA GTG CT |
Dual specificity phosphatase: nested set, 239 bp | GAA GAG AAA GGA CTC AGT GT | AGA TAT ATT TAC AGG ATA GT |
Interleukin 1 receptor, type 1: outer set | TAA AGT TGT CCT GCT TGA GCT GGA | GGC ACG TGA GCC TCT CTT TGC AGT |
Interleukin 1 receptor, type 1, nested set, 197 bp | AAA TCC AAG ACT ATG AGA | CTT AGT GGC TGG TGA CAG T |
Tie-1: outer set | GAA TGT GCT GGT CGG AGA GAA | TGG GGC AGC TTT TCA TAG AGC T |
Tie-1: nested set, 131 bp | AGA GGA GGT TTA TGT GAA GA | ACT ATC TCC CAA AGA AGG ACT |
Small inducible cytokine A18: outer set | TTC TCT GCC TGC CCA GCA TCA TGA | TCA GGC ATT CAG CTT CAG GTC GCT |
Small inducible cytokine A18: nested set, 219 bp | TGT CCT CGT CTG CAC CAT | ATG TAT TTC TGG ACC CAC T |
Psoriasin: outer set | TGA GCA ACA CTC AAG CTG AGA G | TCT CTG GAG GCC CAT TGG T |
Psoriasin: nested set, 238 bp | TTC ACA AAT ACA CCA GAC GTG AT | GGG CGC TGC TCC ATG GCT CTG CT |
Sialoadhesin: outer set | ATT TAG CAG TAG TTC TAT TGG GCA | ACT GAT TAG CAC TTC AGA CGC ACT |
Sialoadhesin: nested set, 171 bp | CTA GTA GTC AGT TGG GAG T | AGC CAG AAC AGC CTT TAC T |