Figure 3
From: Adenoviral gene transfer of angiostatic ATF-BPTI inhibits tumour growth
Biological activity of the encoded proteins. A: Inhibition of the plasmin activity (arbitrary units) by increasing amount of mhATF-BPTI in supernatant of A459 cells infected by Ad Adapt mhAB (black bars) or Ad Adapt Empty (white bar). Values are means of triplicate experiments. Vertical lines indicate SD. B: In vitro assay for endostatin activity. Inhibition of bFGF (1 ng / ml) induced proliferation of HUVEC cells by increasing amounts of hEndostatin in supernatant of A459 cells infected by Ad Adapt hEndo (stripped bars). Ad Adapt Empty infection medium was used as positive control (open bar). Values are means of quadruplicate experiments. Vertical lines indicate SD.