Table 2 Antibodies used in IHC/ICC analysis
Primary antibodies (species) | Clone | Commercial reference | Dilution | Incubation time (min) |
|---|---|---|---|---|
Procollagen 11A1 (mAb) | 1E8.33 | DMTX1/Oncomatrix, Spain | 1:400 | 30 |
Desmin (mAb) | D33 | Dako, Denmark | R-t-U | 20 |
α-SMA (mAb) | 1A4 | Dako, Denmark | R-t-U | 20 |
Vimentin (pAb) | C-20 | Santa Cruz Biotech, Germany | 1:600 | 10 |