Figure 5

Overexpression of miR-9 in normal mouse bone marrow-derived mast cells significantly alters gene expression. Normal mBMMCs transduced with pre-miR-9-3 lentivirus or empty vector control were sorted based on GFP expression. RNA was harvested from mouse BMMCs transduced with empty vector or pre-miR-9-3 lentivirus from three separate transduction experiments. Transcriptional profiling was performed using Affymetrix GeneChip® Mouse Gene 2.0 ST Arrays. Hierarchical clustering was performed for 450 genes differentially expressed (p < 0.05) in mBMMCs expressing either empty vector (EV) or miR-9 (miR9) as determined by one-way ANOVA comparison test (p < 0.05). Mean centered signal intensities of gene-expression are depicted by the log2 of the ratio of the signals against the average signal for each comparison. Color areas indicate relative expression of each gene after log2 transformation with respect to the gene median expression (red above, green below, and black equal to the mean).