Figure 7
From: Identification of oxidized protein hydrolase as a potential prodrug target in prostate cancer
OPH protein levels are differentially expressed in prostate cell lysates. A) RWPE-1, LNCaP, DU 145, and PC3 lysates containing 90 μg of protein were separated by 6% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with anti-OPH antibody. Anti-OPH bands were measured by densitometry and expressed in arbitrary units (A.U.). B) RWPE-1, LNCaP, DU 145, and PC3 lysates containing 90 μg of protein were incubated with 4 mM AcApNA and the amount of p-nitroaniline released after 10 minutes were calculated. PLE (0.5 units) and semi-purified rat liver OPH (250 ng) were used as negative and positive controls respectively. Letters that are not the same are significantly different at P < 0.05.