Figure 2

PI4KA mRNA in in vitro models according to their hepatic differentiation state. Determination by RT-real time PCR of copy numbers of PI4KA transcripts in sub-confluent cultures of Huh-7.5.1 (n = 3) or HepaRG (n = 3) and in normal human liver tissues (n = 31) (A). P values from Mann–Whitney U-tests are indicated. Huh-7.5.1 (B) and HepaRG (C) cell lines were subjected to specific differentiation protocols over several days (see details in Methods section). PI4KA and hepato-specific (albumin and aldolase b) transcripts were assayed by RT-real time PCR at the time points indicated, in three independent experiments. Results are expressed in amounts relative to those at the first time point. The mRNA levels of the succinate dehydrogenase complex, subunit A (SDHA) were used for normalization. The top panels show the comparative expression of PI4KA and albumin. The bottom panels present the correlations between PI4KA and albumin or aldolase B expression levels. Spearman’s rank order coefficients and P values are indicated above the graphs.