Figure 3

Regulation of target protein expression by differentially expressed microRNAs in the ALDH positive MECs of parous and virgin animals. Proteins were extracted from ALDH positive MECs of virgin and parous rat mammary gland. The western blots were repeated atleast thrice for every protein. A) &B) Immunoblots and corresponding densitometry graphs of proteins (n = 6) for some of the differentially regulated genes e.g. ACVR1C, LIFR, CYCLIN E,NOTCH 2, LIN 28, P21 and PPARγ C) Immunoblots and densitometric analysis for EMT related markers like ZEB, SNAIL, N-Cadherin, SLUG, Vimentin and E-Cadherin demonstrate enhanced EMT like characteristics at the translational level in virgin ALDH positive MECs.