Figure 2
Pregnancy alters gene expression profile in ALDH positive MECs. Putative stem cells were obtained from both virgin and parous glands by sorting for ALDHbright population. RNA was extracted and cDNA was prepared post DNAase treatment. PCR array was performed (n = 6) to evaluate the expression status of stem cell associated genes in these cells from the two groups. A threshold of 2 folds up- or down-regulation was considered to generate a dataset of the differentially regulated genes between the two groups. A) Relative expression of all up regulated genes in ALDH positive MECs of parous rats compared to virgin B) Relative expression of all down regulated genes in ALDH positive MECs of parous animals compared to virgin C) &D) Volcano plot of gene expression between parous and virgin ALDH positive MECs, demonstrating the most significantly differentially expressed genes in two different arrays.