Figure 2

NADPH oxidase activity modulated the expression of CTGF and α-SMA. A. Inhibition of NOX activity decrease CTGF and α-SMA expression. Time-course expression of CTGF and α-SMA mRNA evaluated by qPCR in 5 ng/ml TGF-β1- stimulated RMF-EG cells pre-treated or not with DPI. B. DPI inhibited TGF-β1-induced CTGF and α-SMA protein expression. RMF-EG cells were treated with TGF-β1 (5 ng/ml) in the presence or absence of 2,5 μM DPI. Protein expression of CTGF and α-SMA was evaluated by western blot after 24 h and 72 h, respectively. C. Densitometric analysis of western blots bands in B. Columns represent the mean of α-SMA to Tubulin signal and CTGF to Actin signal from three independent experiments; bars ± SE D. DPI inhibits TGF-β1-induced 3TP-luc luciferase reporter activity. RMF-EG cells transiently transfected with 3TP-luc luciferase construct and were pre-treated or not with DPI before stimulation with TGF-β1 for 16 h. Values are average of three separate experiments performed in triplicate.