Figure 3

uPAR is stabilized by HSP70 and MRJ. Knockdown of HSP70 or MRJ promotes uPAR degradation which is rescued by inhibition of proteasome. The HCT116 mock cells were transfected with the indicated amount of psiHSP70 (A) or psiMRJ (B) alone or in combination with 10 μM MG-132. Significant degradation is noted for uPAR in the psiHSP70 or psiMRJ only treated cells. The same blot was probed with antibody against β-actin for comparison.