Figure 3

Cisplatin treatment down-regulates ASS1 protein in HCC cell lines. Cells were treated with indicated cisplatin concentrations in identical rows of a 96-well microplate. After 72 h, triplicate samples were assessed for cell viability by reading the luminescence and lysates were made out of the remaining identical rows. Luminescence values were used to load equal amounts of protein and ASS1 expression was assessed by western blot. ASS1 levels were normalized to GAPDH at each cisplatin concentration and expressed relative to zero drug (100%). The data are representative of three to four independent experiments. Error bars represent S.D. An unpaired t-test was conducted to determine the significance of the change in ASS1 protein levels after each cisplatin concentration treatment as compared to the untreated, or zero drug sample (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001). (A) HepG2 cells. (B) HCC36 cells. (C) SNU182 cells.