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Figure 2 | BMC Cancer

Figure 2

From: Down-regulation of argininosuccinate synthetase is associated with cisplatin resistance in hepatocellular carcinoma cell lines: implications for PEGylated arginine deiminase combination therapy

Figure 2

Methylation status of the ASS1 promoter in HCC cancer cell lines. DNA bisulfite conversion was performed directly from the cells and MSP was subsequently carried out to determine the methylation status of the ASS1 promoter. UM denotes unmethylated and M denotes methylated. Unmethylated and methylated controls (UC and MC) were included to assess bisulfite conversion efficiency. Expected band sizes are as follows: ASS1: 188 bp; control DNA: 274 bp. Data is representative of 3-4 independent experiments. (A) Control: ovarian cell lines A2780 and A2780CR. (B) ASS1-negative/low cells: SNU398, Sk-Hep1 and Tong. (C) ASS1-positive cells: HCC36, SNU182, Malhavu and HepG2.

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BMC Cancer

ISSN: 1471-2407

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