Figure 3
From: MiR-181b sensitizes glioma cells to teniposide by targeting MDM2
MDM2 is a downstream target of miR-181b. A: The mRNA level of MDM2 in miR-181b overexpressed U87 cells was reduced. The protein levels of total MDM2 and phospho-MDM2 were all reduced in miR-181b overexpressed cells compared with vector control (p < 0.01). B. MiR-181b could bind to the 3’-UTR region of MDM2 (3491 to 3497), while the binding was interrupted with mutated MDM2. C: Dural luciferase reporter assay confirmed that miR-181b mimic binds to the 3’-UTR region of wild type MDM2 but not to the mutated form (p < 0.05).