Figure 5
From: Variable NF-κB pathway responses in colon cancer cells treated with chemotherapeutic drugs
Differential NF-κB pathway activation in response to CPT treatment of SW480 cells. A: Reporter SW480 or HCT116 cells were treated with vehicle (DMSO) or with TNFα (100 ng/ml), CPT (1 μM for SW480 and 0.05 μM for HCT116 cells), phleomycin (SW480 only, 100 μg/ml), or erlotinib (HCT116 only, 20 μM). Upper panels show relative NF-κB reporter activity (Y-axis) plotted against the treatments (Y-axis); lower panels show relative amounts of secreted CXCL8 in culture supernatants from treated cells. B: In SW480 and HCT116 reporter cells, the NF-κB target genes, cIAP2 or Bcl-XL, are up-regulated by TNFα and other drugs, but not by CPT. Parental SW480 or HCT116 cells were treated with the indicated drugs as in panel A, and the expression of Bcl-XL and cIAP2 was analyzed by immunoblotting. Tubulin bands are shown as a loading control.