Figure 6
From: ΔNp63α enhances the oncogenic phenotype of osteosarcoma cells by inducing the expression of GLI2
GLI2 mediates the oncogenic potential of ΔNp63α in OS cells. A. Cell lysates of SaOS-2-EV and SaOS-2-ΔNp62α cells untreated or treated with 20 μM GANT61 were analysed using western blotting with an anti- GLI2 antibody. B. GANT61 (20 μM) reversed enhanced anchorage-independent growth in SaOS-2-ΔNp62α cells determined using a soft agar assay and (C) proliferation was assessed using a WST assay. Data represent the mean ± SE of three independent experiments. *P value < 0.05. D. SaOS-2-EV, SaOS-2-ΔNp62α and 143B cells were treated with 20 μM GANT61 and subjected to cell cycle analysis 24 h after drug treatment. SaOS-2-ΔNp62α and 143B cells exposed to GANT61 were arrested in G1 phase. E. Real time PCR analysis of mRNA expression of cell-cycle related genes in SaOS-2-EV and SaOS-2-ΔNp62α cells. 24 h treatment with GANT61 reduced the levels of CyclinD1 and SKPC1 but not n-MYC. *P value < 0.05 (n = 3).