Figure 2From: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer API5 role in cell proliferation and colony formation of HEK293, CaSki, and HeLa cell lines. (A) API5 protein expression was analyzed by western blot. (B) Proliferation assay: 2 × 104 cells were plated in 24 well plates and cultured for additional 6 days. Cells were collected by trypsinization at the indicated times, and live cells were counted after trypan blue staining under a haemocytometer. (C) Colony formation: 500 cells were plated in 6 well plates and cultured for 2 weeks and formed colonies were stained with crystal violet. Data depicted as mean + s.e.m. from one representative experiment performed in triplicate.Back to article page