Figure 8
From: Hypoxia represses microRNA biogenesis proteins in breast cancer cells
Hypoxic regulation of other miRNA biogenesis proteins. Expression of miRNA biogenesis proteins Drosha, TARBP2, DGCR8 and XPO5 were examined under hypoxia vs. normoxia A, Drosha mRNA expression in SKBR3 cells after hypoxia (0.1% O2 48 h) vs. normoxia (P = 0.05). B, TARBP2 mRNA expression in SKBR3 cells after hypoxia (0.1% O2 48 h) vs. normoxia (P = 0.03). *denotes P < 0.05 compared with parallel controls. Data represent normalized mean ± S.E (error bars) (n = 3). mRNA levels were analysed by RT-PCR and normalised to 18S rRNA levels. C, Drosha, TARBP2, DGCR8 and XPO5 protein expression in SKBR3 cells after hypoxia (0.1% O2 48 h) vs. normoxia. Results show three technical replicates per treatment. Protein levels were examined by immunoblotting. α-actinin and β-tubulin used as the loading controls.