Figure 5

Low concentration of DIM induces MCF7 proliferation in absence of E2. (A) p21 expression was quantified in MCF7 cells grown in estrogen-free media for three days and then treated with DMSO, 10 μM DIM or 50 μM DIM for 24 h. (B) Proliferation assay of MCF7 cells, first grown in estrogen-free media and then treated (DMSO, ER, DIM 10 μM or 50 μM) over three days. (C) Proliferation assay of MCF7 cells treated with 10 μM DIM with or without ICI 182 780. (D) Proliferation assay of MCF7 cells treated with 10 μM DIM with or without H89.