Figure 2

ERα degradation increases CYP1A1 induction in response to DIM. (A) Expression analyses were performed in MCF7 cells grown in estrogen-free media for three days and treated with 50 μM ICI 182 780 for 24 h prior to the addition of 50 μM DIM for 24 h. ChIPs of AhR (B) and ERα (C) were performed in MCF7 cells, grown in estrogen-free media for three days and then treated or not with 50 μM ICI 182 780 for 24 h prior to the addition of 50 μM DIM for 1 h. Results are shown as% of Input and represent the mean of three independent experiments with standard deviation.