Figure 1

DIM activates both AhR and ER signaling pathways. (A) MCF7 cells, grown in estrogen-free media for three days, then treated with DMSO, 10nM TCDD, 10nM TCDD + 100nM E2, 50 μM DIM or 50 μM DIM + 100nM E2. After 24 h, the cells were lysed and RNA was extracted and quantified by RT-qPCR. Results are presented as percent induction over TCDD. (B) Schematic representation of the CYP1A1 promoter and primer position used for ChIP analysis. ChIPs of AhR (C) and ERα (D) were performed in MCF7 cells, grown in estrogen-free media for three days, then treated with DMSO, 10nM TCDD, 10nM TCDD + 100nM E2, 50 μM DIM or 50 μM DIM + 100nM E2. Results are showed as% of Input and represent the mean of three independent experiments with standard deviation.