Figure 6
From: IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells
Induction of macrophage cytoskeletal changes by Wnt5a-conditioned medium. Wnt5a-conditioned medium from Wnt5a-transfected BGC-803 cells induced macrophage cytoskeletal changes by FITC-phalloidin and DAPI staining, which was inhibited by the addition of MCP-1 neutralizing antibody AF-479-NA (0.1 μg/ml) into the medium or the pretreatment of Wnt5a-transfected BGC-803 cells with rIL-1Ra. Wnt5a V: Wnt5a vector; rIL-1Ra: recombinant IL-1 receptor antagonist.