Figure 3
From: IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells
Induction of IL-1β expression by Wnt5a in gastric cancer cells. (A) and (B) Wnt5a transfection induced IL-1β and TNF-α expression in BGC-803 cells by real-time PCR and ELISA. (C) Wnt5a transfection had no effect on IL-6 expression in BGC-803 cells. (D) Wnt5a transfection stimulated IL-1β expression in HGC-27 cells. (E) and (F) Wnt5a transfection had no effect on TNF-α and IL-6 expression in HGC-27cells. (G) Wnt5a siRNA inhibited IL-1β expression in MKN-45 cells. (H) Wnt5a siRNA has no effect on TNF-α expression in MKN-45 cells. *P < 0.01 vs Control. Control V: control vector; Wnt5a V: Wnt5a vector. Control si: control siRNA; Wnt5a si: Wnt5a siRNA. Data are expressed as mean ± SD, n = 3.