Figure 4

Vaccine co-expression IL-18 and GM-CSF treatment increased local expression of IL-18, GM-CSF, and IFN-γin vivo. Serum from each group including non-immunized group was collected through caudal vein on 2 day, 4 day, 6 day and 8 day after the third immunization respectively. ELISA was carried out to detect the level of IL-18 (A), GM-CSF (B), IFN-γ (C), TGF-β (D),TNF-α (E) and IL-10 (F) in serum respectively. Experiments were performed in triplicate and repeated three times. Each data point indicates means ± SD. Vaccine co-expression IL-18 and GM-CSF produced a synergistically higher levels of IL-18, GM-CSF and IFN-γ than LL/2 control group (P < 0.01, n = 7). TGF-β showed no difference in all groups (P > 0.05, n = 7).