Figure 1From: The role of RhoC in epithelial-to-mesenchymal transition of ovarian carcinoma cells The involvement of RhoC in EMT of ovarian carcinoma cells. The mRNA and protein expression of RhoC was screened in ovarian carcinoma cells (SKOV3, OVCAR3, CAOV3, HO8910 and ES-2) by real-time PCR (A) and Western blot (B). CAOV3 cells were transfected with RhoC-expressing plasmid and confirmed by real-time PCR and Western blot (C). After transfection of RhoC siRNA, RhoC expression became weaker in OVCAR3 by real-time PCR and Western blot (D). CAOV3 cells became spindle after ectopic RhoC expression, while RhoC knockdown caused OVCAR3 morphorlogically round (E). There was a down-regulated expression of E-cadherin mRNA, and up-regulated expression of N-cadherin and a-SMA mRNA in CAOV3 transfectants by real-time PCR (F). After the treatment of RhoC siRNA, there was an increased expression of E-cadherin mRNA in OVCAR3 cells by real-time PCR, while the converse was true for the expression of N-cadherin and a-SMA mRNA (F). * compared with control and mock, p < 0.05.Back to article page