Figure 4From: Inhibitory effects of prostaglandin E2on collagen synthesis and cell proliferation in human stellate cells from pancreatic head adenocarcinoma Effect of different agonists on DNA synthesis and phosphorylation of ERK and Akt in pancreatic stellate cells. A. Effect of PDGF (10 ng/ml), EGF (10 nM), and TGFβ (10 ng/ml) on DNA synthesis. Cells in serum-free medium were stimulated for 24 hours, with [3H] thymidine added at 18 hours. DNA synthesis was assessed as described in Methods. Results are presented as mean +/−SEM of six experiments. B. Effect of pretreatment with indomethacin (10 μM) for one hour before stimulation of cells with PDGF for 24 hours. Results are presented as mean +/−SEM of three experiments. C. Effect of PDGF (10 ng/ml), EGF (10 nM), and TGFβ (10 ng/ml) on phosphorylation of Akt and ERK. Cells in serum-free medium were stimulated for 5 minutes before harvesting and analysis of cell lysates on Western blots. Blots are from one typical of four experiments. Histograms represent mean +/−SEM of four experiments. D. Effect of thrombin (1 U/ml), PGE2 (10 μM), fluprostenol (10 μM), and forskolin (10 μM) on ERK phosphorylation. Cells were stimulated for 5 minutes before harvesting. Blots from one typical of four experiments are shown. Histograms represent mean +/−SEM of four experiments. * Sign. different from control.Back to article page