Figure 3

Phenotype of DU145-LN cells with increased metastatic ability. (A) Phase contrast microscopy images of parental DU145 cells, DU145-LN1, DU145-LN2, and DU145-LN4 cells. Cells exhibit progressive phenotypic changes after selection, with increased clustering and cell-cell adhesions from parental DU145 (top left panel) to DU145-LN4 (bottom right panel). Insets are larger images of lower panels. Scale bar = 0.5 mm (B) DU145-LN4 tumor cells maintain their mesenchymal-epithelial transition (MET) phenotype in vivo. IHC of DU145 and DU145-LN4 subcutaneous tumor tissue with the epithelial markers, E-cadherin and EpCAM. High expression of E-cadherin and EpCAM was maintained in the tumor tissue. Scale bar = 100 μm.