Figure 4
From: Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells
The effect of ADHFE1 down regulation on cell viability and proliferation. The cell viability and proliferation of HT-29, SW480, and DLD-1 cells after ethanol treatment, transfection of ADHFE1 siRNA and combined treatment are determined by MTT, cell counting, and counter staining assay. A. Cell viability of 3 CRC cells is significantly increased by treatment with ethanol, siRNA, and combination of both. B. Cell proliferation of 3 CRC cells is significantly increased by ethanol, siRNA, and co-treatment. C. The captured images of cells using Hoechst 33342 show that the number of 3 CRC cells is increased by ethanol, siRNA, and co-treatment. D. The mRNA expression of ADHFE1 is significantly decreased in 3 CRC cells by treatment with ethanol, siRNA, and combination of both. E. ADHFE1 protein expression is decreased in 3 CRC cells by treatment with ethanol, siRNA, and combination of both. GAPDH was used as a loading control. *p-Values of < 0.05 were considered statistically significant. +: Treated with agent; −: Non-treated with agent.