Figure 4
The effects of taxanes on the microtubule formation, M-phase arrest and cell apoptosis on the selected MCF-7 cell lines. (A-B), MCF-7CC, MCF-7TXT and MCF-7DOX cells were seeded on coverslip and treated with docetaxel (A) and paclitaxel (B) of indicated concentrations for 24 hours. The microtubule formation, M-phase arrest and cell apoptosis were determined by fluorescence microscopy. The red indicates the α-tubulin and the blue is the DAPI stain for DNA. Size bar: 10 μm. (C&D) The effects of docetaxel (C) and paclitaxel (D) on the apoptosis of MCF-7CC, MCF-7TXT and MCF-7DOX cells. The apoptotic cells were determined by chromatin condensation and nuclear morphology as revealed by DAPI stain in A &B. For each data, 300 cells from at least three independent experiments were examined. The error bar is the standard error.