Figure 3

The effect of DDR2 mutation on lung SCC cells proliferation in vitro. HBE, H1703 and SK-MES-1 cells were transfected with pEGFP-DDR2, pEGFP-DDR2-S131C, pEGFP-DDR2-T681I or empty vector. (A, B, C) An MTT assay was performed to determine the proliferation of pEGFP-DDR2, pEGFP-DDR2-S131C, pEGFP-DDR2-T681I or empty vector-transfected HBE, H1703 and SK-MES-1 cells. (D,E) A colony-forming growth assay was performed to determine the proliferation of pEGFP-DDR2, pEGFP-DDR2-S131C, pEGFP-DDR2-T681I or empty vector-transfected HBE, H1703 and SK-MES-1 cells. The colonies were counted and captured.