Figure 4

MiR-133b direct targets Gli1 in GC cells. (A) Sequence of the Gli1 3′UTR showing the miR-133b binding seed region and mutation of the Gli1 3′UTR seed region to create Gli1-mut. (B) Luciferase activities of the reporter containing wild-type Gli1 3′UTR or mutant Gli1 3′UTR are shown in the bar graph. (C) Relative mRNA levels of Gli1 in indicated cells analyzed by qRT-PCR is shown in the bar graph. (D) Representative western blot images of indicated protein in MKN-28 cells (left panels), with relevant quantification (right panel). Relative mRNA levels of Zeb2 (E) and OPN (F) in indicated cells analyzed by qRT-PCR are shown in the bar graph. The results are the mean of three independent experiments ± S.D. *, p <0.05.