Figure 2
MMP-10 expression in HeLa and UROtsa human cell lines. A, MMP-10 expression was assessed by quantitative reverse transcriptase-PCR (qPCR), and immunoblot (IB) analyses in HeLa and UROtsa cells. MMP-10 activity was assessed by zymography, demonstrated by the degradation of casein. B, MMP-10 expression and activity was assessed by qPCR, immunoblot analyses and zymogram in stable HeLa clones; HeLa-MMP-10OE overexpressing a functionally human MMP-10 in a pCMV6-Entry vector, and HeLaEmpty an empty-vector transfected control. C, MMP-10 assessed by qPCR, immunoblot and zymography in UROtsa clones; UROtsa-MMP-10KD-1, UROtsa-MMP-10KD-2 functional knockdown clones transfected with MMP-10 siRNA, and UROtsa-MMP-10Scr transfected with a scrambled siRNA control.