Figure 4

NEU-induced activation of DNA damage response pathway is cell cycle phase dependent. (A) HeLa cells were treated with 10 mM NEU for different time intervals and lysates were analysed for activation of checkpoint proteins by immunoblotting. (B) HeLa cells synchronised in S phase using double thymidine block were treated with 10 mM NEU for different time intervals and lysates were analysed for activation of checkpoint proteins by immunoblotting. (C) HeLa cells synchronised in G2 phase using thymidine-nocodazole block were treated with 10 mM NEU for different time intervals and lysates were analysed for activation of checkpoint proteins by immunoblotting. (D) Cell cycle profile of propidium iodide (PI) stained asynchronous, S phase synchronised and G2-M phase synchronised HeLa cells using Flow Cytometer.