Figure 2

Transfection, Lenti-virus production and transduction. Co-transfection of the Trans-Lentiviral packaging mix with a shRNA transfer vector into HEK293T packaging cells was done using Arrest-In Transfection Reagent. Following co-transfection, replication-incompetent virions were released into the media which were collected after 48 h and 72 respectively. (A) Fluorescence microscopy image showing TurboGFP expression from the pGIPZ STMN1 vector cells 72 h post-transfection. (B) Image showing the TurboGFP expression from the GIPZ Non-silencing Control. (C) Phase contrast microscopy image. (D) TurboGFP images showing shRNA delivery efficiency. (E) &(F) post puromycine selection images showing efficient transduction of Lenti-STMN1 shRNA in distal esophageal cancer cells.