Figure 6From: BIMELis a key effector molecule in oxidative stress-mediated apoptosis in acute myeloid leukemia cells when combined with arsenic trioxide and buthionine sulfoximine Silencing of BIM EL with si RNA abolishes ATO/BSO-induced cell death. The expression of BIMEL and cleavage of caspase 3 and PARP were determined by immunoblotting. HL60 cells were transfected with two siRNAs designed against BIMEL (BIM#1 and BIM#2) or control siRNA, incubated for 48Â h, and treated with ATO/BSO for 12Â h. A typical result of 3 independent experiments is shown. IP, immunoprecipitation; Ppt, immunoprecipitate.Back to article page