Figure 5
BSO induces the dissociation of phosphorylated BIM EL from MCL1 and the interaction with BAX. A and B, The dissociation of phosphorylated BIMEL and MCL1, and the interaction with BAX were determined by immunoprecipitation and immunoblotting with antibodies to their normal and phosphorylated forms. Values were normalized to actin or IgG(L), respectively and represent relative changes compared with control. A typical result of 3 independent experiments is shown. IP, immunoprecipitation; Ppt, immunoprecipitate; Sup, supernatant.