Figure 5

Bone and lung lysates from mice treated with anti-IL-17A antibody are less chemotactic than their control antibody treated counterparts. A and B) Western blotting showing the presence of CXCR4 on the tumors derived from A) 4 T1 tumor bearing SKG mice and B) PyV MT-arthritic mice treated with control or anti-IL-17A antibody (N = 3 tumors). C and D: In vitro trans-well invasion assay using 4 T1 cells in the upper chamber and lung (C) or bone (D) lysate from 4 T1 tumor bearing SKG mice treated with control or anti-IL-17A antibody in the lower chamber. E and F: In vitro trans-well invasion assay using PyV MT tumor cells in the upper chamber and lung (E) or bone (F) lysate from arthritic PyV MT mice treated with control or anti-IL-17A antibody in the lower chamber. Various treatment groups and significance are shown in the figure. *P < 0.05, **P < 0.01, ***P < 0.001 (N = 5 mice). We used a one way ANOVA with Tukey to compare all groups to each other.