Figure 7
DGK ζ Silencing Attenuates Invasion of PC-3 Prostate and MDA-MB-231 Breast Cancer Cells. ( A ) Western blot of DGKζ (arrow) in three PC-3/Vector and three PC-3/shRNA cell lines. Tubulin is shown for comparison. ( B and C ) Graphs showing the average DGKζ level ( B ) and invasion through Matrigel-coated Transwell inserts ( C ) of PC-3/Vector and PC-3/shRNA cell lines. The data were normalized to Empty Vector control cells. Values in B and C are the mean ± S.E.M. from five independent experiments. (D) Western blot of DGKζ in MDA-MB-231/Vector and MDA-MB-231/shRNA cells. Tubulin is shown for comparison. ( E and F ) Graphs showing the average DGKζ level ( E) and invasiveness ( F ) of MDA-MB-231/Vector and MDA-MB-231/shRNA cells, normalized to the level in Empty Vector control cells. Values in E and F are the mean ± S.E.M. from four independent experiments. An asterisk indicates a significant difference (P < 0.05) from Vector cells by Student’s t-test.