Figure 3

MV-hE:A and MV-mE:A conditioned media inhibit angiogenic processes. (A) Conditioned media (10 μg/ml total protein) from the MV-E:A viruses, but not MV-GFP inhibits tube formation in bEnd.3 mouse endothelial cells stimulated with 20 ng/ml recombinant mouse VEGF (*p ≤ 0.05, ** p ≤ 0.001). (B) MV-E:A conditioned media suppresses VEGF enhancement of cell viability in HUVEC and bEnd.3 cells (*p ≤ 0.05). (C) An angiogenesis protein array reveals that MV-hE:A conditioned media (100 μg/ml total protein) downregulates multiple angiogenic proteins in HUVECs compared to MV-GFP. Changes in protein expression are quantified in the accompanying bar graph.