Figure 6

Representative data from the proteomics study on MDA-MB-231 cells after knockdown of KIAA1199. A) Differentially expressed proteins were classified based on their Gene Ontology (GO) and function. B) MS and MS2 spectra from one up-regulated protein (S100A11) and two down-regulated proteins (WASL and PPP1R9B). The left-hand panels show MS spectra, where the red *symbols show intensities of the monoisotopic peak for the light and heavy SILAC labeled peptides. The right-hand panels show the MS2 spectra corresponding to the peptide with the most intense signal of the pair. C) Validation of differential expression of S100A11, WASL and PPP1R9B genes using semi-quantitative RT-PCR analysis in triplicate. D) The relative expression of the bands is shown as bars (*p < 0.01). Intensities were normalized to GAPDH band. The average intensity values of the transcripts obtained from the MDA-MB-231-ShNC cells were set to 100%.