Analytical validation of the PAM50-based Prosigna Breast Cancer Prognostic Gene Signature Assay and nCounter Analysis System using formalin-fixed paraffin-embedded breast tumor specimens

Table 6 Comparison of gene expression at different masses from the RNA input study

Mass (ng)	Pairwise (n)	Pearson	Slope	Intercept	ΔROR
Mass (ng)	Pairwise (n)	[95% CI]	[95% CI]	[95% CI]	[95% CI]
62.5	21	0.97 [0.93–0.99]	0.96 [0.91–1.00]	-0.02 [-0.05–0.01]	0.48 [-1.27–2.22]
125	46	0.99 [0.97–0.99]	0.98 [0.96–1.01]	-0.01 [-0.03–0.01]	-0.04 [-0.89–0.8]
250	23	0.99 [0.98–1.00]	1.00 [0.99–1.01]	0.00 [-0.01–0.01]	-0.39 [-0.96–0.17]
500	46	0.99 [0.99–1.00]	0.97 [0.96–0.99]	0.02 [0.01–0.04]	-0.57 [-1.39–0.26]
625	23	0.99 [0.98–1.00]	0.95 [0.92–0.99]	0.03 [0.01–0.06]	-0.78 [-2.2–0.63]

Pairwise correlations, slopes, and intercepts of normalized 50 genes and change in ROR score for replicate RNA Hybridizations with different mass inputs. The average intercept, slope, Pearson’s correlation, and change in ROR for the pair-wise comparisons are reported with their 95% confidence intervals.

ISSN: 1471-2407